Conservation of the native species in the Magnoliaceae: micropropagation and mycorrhization techniques  

Project Title: Conservation of the native species in the Magnoliaceae: micropropagation and mycorrhization techniques

Applicant: Hong Kong Polytechnic University

Total Approved Grant: $480,164.17 (jointly funded by ECF and WWGF on an equal share basis)

Duration: 1.6.2000 - 31.5.2003

Project Status/Remarks: Completed

Scope:
Conservation of the native species in the Magnoliaceae: regeneration of plantlets by micropropagation techniques and production of mycorrhizal seedlings suitable for planting out.

Summary of the Findings/Outcomes :

Four species in the Magnoliaceae family (Micheria macclurei, M. chapensis, M. maudiae and Maglietia fordiana) have been investigated initially. Finally it has boiled down to M. macclurei only, because materials from this species were comparatively abundant for carrying out the necessary experiments. A total of 43 separate primary culture experiments and more than 62 subculture experiments were done during the period of the project. The experience and the protocols developed in the above investigation are serving as significant path stones for further studies of micropropagation of the tree species in Magnoliaceae. Seedlings of Micheria maccclurei have been successfully regenerated from seed tissue using tissue culture technique. Also a considerable amount of plantlets will be proliferated from these seedlings for out-planting. 11 types of arbuscular mycorrhizal fungi have been identified from the trap pot cultures using rhizospheric soils of the studied Magnoliaceae species. Plantlets of M. macclurei developed from the seed tissue have been transferred to the AFCD for out-planting and inoculation in Hong Kong.